组织工程实用工具: 再生疗法的设计与评价课程详细信息

课程号 00333126 学分 3
英文名称 The Tissue Engineer’s Toolkit: Design and Evaluation of Regenerative Therapies
先修课程
中文简介 组织工程/再生医学要求能够控制诸如增殖、迁移和分化之类的细胞行为。本课程将为工程专业的学生介绍以下两套工具:(1)现有治疗工具,包括可溶性生长因子、不可溶黏附配体、支架拓扑结构和外部施加机械力;(2)能评估细胞和组织对治疗手段的反应的实验工具,包括高通量基因组分析、时定量多聚酶链反应、酶联免疫吸附测定、免疫印迹法、免疫组织化学着色、能确认治疗机理的失活技术。
英文简介 Tissue engineering/regenerative medicine requires the capability to regulate cellular behaviors such as proliferation, migration, and differentiation. This course will introduce engineering students to (1) the therapeutic tools we have available for this purpose, including soluble growth factors, insoluble adhesion ligands, scaffold topographic features, and externally applied mechanical forces and (2) the experimental tools to evaluate cellular and tissue responses to therapeutic treatment including high throughput genomic analysis, quantitative real time polymerase chain reaction, ELISA, Western blotting, immunohistochemical staining, and loss of function techniques to confirm therapeutic mechanisms.
开课院系 工学院
通选课领域  
是否属于艺术与美育
平台课性质  
平台课类型  
授课语言 英文
教材
参考书
教学大纲 At the end of the course, students will be able to design biomaterial scaffolds using a wide range of
tissue engineering tools and design experiments with appropriate controls to assess their efficacy.
Tissue engineering/regenerative medicine requires the capability to regulate cellular behaviors such as proliferation, migration, and differentiation. This course will introduce engineering students to 1) the therapeutic tools we have available for this purpose, including soluble growth factors, insoluble adhesion ligands, scaffold topographic features, and externally applied mechanical forces and 2) the experimental tools to evaluate cellular and tissue responses to therapeutic treatment including high throughput genomic analysis, quantitative real time polymerase chain reaction, ELISA, Western blotting, immunohistochemical staining, and loss of function techniques to confirm therapeutic mechanisms.

Topics
1. Introduction-the motivation and conceptual framework of tissue engineering / regenerative
medicine.
2. Soluble cues-growth factor activity, receptors, intracellular signaling, and the promise and
challenge of therapeutic application.
3. Substrate cues-adhesion ligands and scaffold structural features
4. Mechanical cues-mechanobiology, substrate stiffness, and external loads
5. Cell therapy-choices, benefits, and challenges
6. High throughput transcriptional profiling
7. Quantitative real time polymerase chain reaction-theory, experimental design, and quantitative
analysis.
8. Protein analysis-antibodies, Western blotting, ELISA, immunohistochemistry.
9. Mechanistic tools-function-blocking antibodies, chemical inhibitors, and RNA interference

Course handouts and selected articles from peer-reviewed literature provided by the instructor.
课堂讲授 60%
课堂报告 30%
课堂讨论 10%
Four Quizzes   20%
Midterm Exam   25%
Proposal  15%
Final Exam  30%
Attendance and Participation  10%
Total 100%
教学评估 Ken Webb:
学年度学期:17-18-3,课程班:组织工程实用工具: 再生疗法的设计与评价1,课程推荐得分:null,教师推荐得分:null,课程得分分数段:80及以下;
学年度学期:18-19-3,课程班:组织工程实用工具: 再生疗法的设计与评价1,课程推荐得分:null,教师推荐得分:null,课程得分分数段:80及以下;